Injectoporation
Webb29 mars 2024 · Culture and injectoporation of cochlear explants. Injectoporation of cochlear explants was performed following recently established protocols (Xiong et al., 2014). In brief, cochleae were dissected from postnatal (P) day 4 and P5 wild-type mice, cut into three pieces, and cultured for 4 h in DMEM/F12 medium with 10 ng/μl ampicillin. Webb14 okt. 2024 · Injectoporation was performed as previously described (Xiong et al., 2014). In brief, the otic vesicles from mice between postnatal day 0 (P0) and P8 were dissected from the skull, and the bony capsules were removed and the Corti organ was exposed. The remaining cochlear ducts were transferred to the lid of a 35-mm Petri dish.
Injectoporation
Did you know?
WebbInjectoporation allows for gene transfer into dozens of hair cells, and it is compatible with the analysis of hair cell function using imaging approaches and electrophysiology. … WebbInjectoporation in inner ear. Research article PDZD7-MYO7A complex identified in enriched stereocilia membranes Clive P Morgan et al. DOI: 10.7554/eLife.18312 Your …
WebbArticle Title: Using injectoporation to deliver genes to mechanosensory hair cells. doi: 10.1038/nprot.2014.168. Figure Lengend Snippet: Injectoporation setup. ( a ) Diagram of the injectoporation setup. ( b ) Left, image of the injectoporation setup. Note that the electroporator is not shown.
WebbSpecial Technique: Nano-injection (PSCC), injectoporation (pDNA delivery to culture tissue), Hearing test (ABR(auditory brainstem response) test), Gene delivery (various nanoparticles), CRISPR mouse… 더보기 Studying about inner ear hair cells of mechanotransduction and regeneration. Webb15 maj 2024 · Using injectoporation, we expressed the cDNAs in OHCs from Tomt add/add mice at P3 and analyzed transducer currents after one day in culture (Figure 7O–Q). OHCs from the mid-apical region of the cochlea expressing the cDNAs were identified by GFP fluorescence and mechanotransduction currents were evaluated by …
WebbHere you will find our spare parts, our tools and equipment for the repairing of Diesel Injectors, Diesel pumps and Turbochargers, the exploded drawings, all Redat products and their applications. In our Diesel catalogues you can find: Denso, Caterpillar Perkins, Bosch, MTU L’Orange, Delphi, Cummins, Siemens and Unit injector.
Webb13 nov. 2024 · Take advantage of our tissue injectoporation system, 121 we electroporated m-3j-gRNA1 and wildtype spCas9 (PX330-m-3j-gRNA1) in 122 postnatal … givenchy oscar dressesWebbFigure 3 Diagram of the microinjection procedure and evaluation of the injectoporation efficiency. (a) The diagram shows a cross-section of the organ of Corti. (b) For gene … givenchy oudWebbInjectoporation of Ripor2-constructs and immunostaining The generation of Ripor2 LacZ/LacZ mice has been described previously. 19 For Ripor2 DNA construct generation, Ripor2 cDNA (NM_029679.2, without exon 13) was amplified from a mouse-cochlear cDNA library and cloned into a pEGFP-N3-derived vector from which the EGFP (enhanced … für was ist bromazanil gutWebbDownload scientific diagram Localization of Smpx in hair cells via in vivo electrotransfection (injectoporation). Red, phalloidin; Green, GFP. (a-c) Hair cells transfected with pEGFP. These ... givenchy oversized shirtWebb2 okt. 2009 · Semantic Scholar extracted view of "Mechanotransduction by Hair Cells: Models, Molecules, and Mechanisms" by P. Gillespie et al. für was ist apixabanWebbTo circumvent this issue, we make use of an organotypic culture approach of early postnatal organs of Corti (Vogl et al., 2015) and have established a microinjection … für was ist aconitumWebbWarzone Free Injector – Easy to Use! Warzone Free Injector supports these game; Warzone. Thanks to this Free Injector, you can easily run .dll cheats. enjoyI’m releasing a 100% undetected Injector. Warzone Injector has a carefully selected set of features in its core library to support many... UNDETECTED. für was ist arnica d12 gut