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Klf5 chip

WebMar 17, 2024 · ChIP with the KLF5 antibody from R&D (#AF3758) was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling, #9003) following the … WebAug 1, 2024 · KLF5 regulates key biological processes in the human airway epithelium To determine the target genes of KLF5 in human lung epithelial cells, we performed RNA-seq on triplicate samples of Calu-3 cells treated with either negative control (NC) siRNA or siRNA specific for KLF5.

Frontiers Krüppel-Like Factors in Metabolic Homeostasis and ...

WebWe performed RNA sequencing, immunohistochemistry, western blotting, real time-PCR, ELISA, luciferase assay, chromatin immunoprecipitation (ChIP), and flow cytometry to demonstrate the effects of KLF5 on CD8 + T cell infiltration and related molecular mechanism. Single-cell RNA sequencing and spatial transcriptomics analysis were … WebFeb 20, 2024 · ChIP-seq data Rogerson et al., 2024 demonstrate that KLF5 strongly binds to the JUP and CCNE1 enhancers but low levels are observed at the MYBL2 enhancer . KLF5 depletion ( Figure 3—figure supplement 1H ) caused reduced expression of all three target genes ( Figure 3H ). horsehead lodge https://akshayainfraprojects.com

Acetylation of KLF5 maintains EMT and tumorigenicity to cause ...

WebOct 15, 2024 · Chromatin immunoprecipitation (ChIP) assays indicate that the Fgf4 locus is occupied by Klf5, suggesting direct regulation of Fgf4 by Klf5. In terms of the emergence of Nanog + pluripotent EPI cells, the phenotypes of Klf5 KO embryos can be reversed by either Fgfr or ERK inhibition. WebAntibodies that detect KLF5 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ChIP and ELISA. These antibodies target KLF5 in Human, Mouse and Rat samples. Our KLF5 polyclonal, monoclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rabbit … WebAntibodies that detect KLF5 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ChIP and ELISA. These antibodies … horsehead lodge arkansas

Klf5 establishes bi-potential cell fate by dual regulation of ICM and …

Category:KLF5 inhibition potentiates anti-PD1 efficacy by …

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Klf5 chip

Klf5 establishes bi-potential cell fate by dual regulation of …

WebFeb 10, 2008 · Interestingly, the ChIP-on-chip data revealed a weak signal of Klf2, Klf4 and Klf5 occupancies at the Fgf5 and Nes promoters. This binding was confirmed using ChIP–qPCR assays, and showed... WebNov 9, 2024 · Klf5 is essential for bi-potential cell fate; a single Klf5 -overexpressing embryonic stem cell (ESC) generates terminally differentiated embryonic and extra …

Klf5 chip

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http://sourcedb.kiz.cas.cn/zw/lw/202404/t20240413_6738169.html WebJan 24, 2024 · ChIP assays were conducted to verify the enrichment of the potential binding sites by KLF5 antibody in BT-549 cells. The ChIP results were analyzed by qPCR and nucleic acid electrophoresis. The dual-luciferase reporter assays were performed to validate the interaction between KLF5 and the first binding site (E1).

WebKLF5 is a Krüppel-like transcription factor broadly involved in development and regeneration whose lineage specificity, if any, remains unclear. Here we report KLF5 specifically marks the epidermis, and its deletion leads to skin barrier dysfunction in vivo. WebSep 7, 2024 · KLF5 is a member of the Kruppel-like factor, subfamily of zinc finger proteins that are involved in cancers. KLF5 functions as a transcription factor and regulates the …

WebFeb 21, 2024 · Krüppel-like Factor 5 (KLF5) is a cell-intrinsic oncogene to promote tumorigenesis. However, the cell-extrinsic effects of KLF5 on suppressing the immune … WebOct 22, 2009 · The Krüppel-like factor 5 (KLF5) is a zinc-finger transcription factor promoting cell proliferation, cell-cycle progression and survival. A high expression level of KLF5 mRNA has been shown to be associated with shorter breast cancer patient survival. However, the mechanism of KLF5 action in breast …

WebMar 21, 2024 · KLF5 (KLF Transcription Factor 5) is a Protein Coding gene. Diseases associated with KLF5 include Cardiomyopathy, Dilated, 1Ff and Sigmoid Disease.Among its related pathways are White fat cell differentiation and RNA Polymerase I Promoter Opening.Gene Ontology (GO) annotations related to this gene include DNA-binding …

WebNational Center for Biotechnology Information psig code of best practiceWebOct 7, 2024 · To determine direct gene targets of KLF5, we defined the cistrome of KLF5 using ChIP-seq in both Calu-3 and 16HBE14o- lung epithelial cells lines. Occupancy site concordance analysis revealed that KLF5 co-localized with the active histone modification H3K27ac and also with binding sites for CCAAT Enhancer Binding Protein Beta (C/EBPβ). psig atmosphereWebJan 13, 2024 · Mechanistically, YB-1 enhances KLF5 expression not only through transcriptional activation that can be inhibited by DACH1, but also by stabilizing KLF5 … horsehead lodge azWebNov 9, 2024 · Klf5 is essential for bi-potential cell fate; a single Klf5-overexpressing embryonic stem cell (ESC) generates terminally differentiated embryonic and extra … psig chicagoWebOverview. A cell-permeable phenylpropenoate compound that inhibits the proliferation of colon cancer cell lines exhibiting high levels of KLF5 (IC 50 = 1.6 µM in DLD-1 cells). … horsehead lodge clarksville arWebSep 7, 2024 · Overall, five samples of KLF5 ChIP-Seq datasets were obtained, plus PCGs and lncRNAs with a KLF5 binding site in at least two samples were selected for the first step. Second, the differently expressed lncRNAs and PCGs were obtained from the intersection results of the RNA-Seq dataset (GSE50760) and Cancer RNA-Seq Nexus database. ... horsehead logoWebHIF-1α活化对肾小管间质炎症和纤维化的影响及其机制探讨.pdf psig combating pension scams