WebMar 17, 2024 · ChIP with the KLF5 antibody from R&D (#AF3758) was performed using the SimpleChIP® Enzymatic Chromatin IP Kit (Cell Signaling, #9003) following the … WebAug 1, 2024 · KLF5 regulates key biological processes in the human airway epithelium To determine the target genes of KLF5 in human lung epithelial cells, we performed RNA-seq on triplicate samples of Calu-3 cells treated with either negative control (NC) siRNA or siRNA specific for KLF5.
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WebWe performed RNA sequencing, immunohistochemistry, western blotting, real time-PCR, ELISA, luciferase assay, chromatin immunoprecipitation (ChIP), and flow cytometry to demonstrate the effects of KLF5 on CD8 + T cell infiltration and related molecular mechanism. Single-cell RNA sequencing and spatial transcriptomics analysis were … WebFeb 20, 2024 · ChIP-seq data Rogerson et al., 2024 demonstrate that KLF5 strongly binds to the JUP and CCNE1 enhancers but low levels are observed at the MYBL2 enhancer . KLF5 depletion ( Figure 3—figure supplement 1H ) caused reduced expression of all three target genes ( Figure 3H ). horsehead lodge
Acetylation of KLF5 maintains EMT and tumorigenicity to cause ...
WebOct 15, 2024 · Chromatin immunoprecipitation (ChIP) assays indicate that the Fgf4 locus is occupied by Klf5, suggesting direct regulation of Fgf4 by Klf5. In terms of the emergence of Nanog + pluripotent EPI cells, the phenotypes of Klf5 KO embryos can be reversed by either Fgfr or ERK inhibition. WebAntibodies that detect KLF5 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ChIP and ELISA. These antibodies target KLF5 in Human, Mouse and Rat samples. Our KLF5 polyclonal, monoclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rabbit … WebAntibodies that detect KLF5 can be used in several scientific applications, including Western Blot, Immunocytochemistry, Immunohistochemistry, ChIP and ELISA. These antibodies … horsehead lodge arkansas